Validation of a Prion and Virus Purification Process in the Manufacture of Bovine Thrombin
نویسنده
چکیده
Background: To further minimize transmission risk of blood-borne viral and prion diseases, manufacturers of therapeutic animalor human-derived proteins have implemented improved in-process pathogen-removal procedures. Objectives: To evaluate removal of transmissible spongiform encephalopathy agents (prions) and viruses using Omega 100K VR membrane filtration system (Pall Filtron, Northborough, MA) during manufacture of bovine thrombin. Methods: Purified bovine thrombin was challenged with a high-titer clarified brain homogenate of the 263K hamster scrapie strain, a model for bovine transmissible spongiform encephalopathy agents, and 4 representative model viruses: bovine parvovirus (BPV), xenotropic murine leukemia virus (XMuLV), pseudorabies virus (PRV), and bovine viral diarrhea virus (BVDV). Using a
منابع مشابه
Purification and Partial Characterization of a Thrombin-Like Enzyme (AH144) from Venom of Iranian Snake Agkistrodon Halys
The snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. Purification and partial characterization of a thrombin-like enzyme from the venom of the Iranian snake, Agkistrodon halys, was the aim of this study. Purification was carried out by a combination of variety of chromatographic methods that included: gel...
متن کاملPolymorphism of Prion Protein Gene (PRNP) in Iranian Holstein and Two Local Cattle Populations (Golpayegani and Sistani) of Iran
Bovine spongiform encephalopathy (BSE) is a fatal infectious neurodegenerative disease in cattle, characterized by the accumulation of an abnormal, proteaseresistant prion protein (PrPSc) in the brain. BSE is similar to scrapie in sheep and goats and Creuzfeldt-Jakob disease in humans. Susceptibility in cattle hasbeen shown to be under the influence of two polymorphic locations, which are...
متن کاملComparison of two gel filtration chromatography resins for the purification of foot-and-mouth disease virus as a purified vaccine antigen
Introduction: Foot-and-mouth disease (FMD) is a highly contagious and economically devastating viral disease of livestock that is categorized in list A of animal diseases by the World Organisation for Animal Health (OIE). Vaccination is effective against FMD and the vaccine production centers largely use the industrial ultra-filtration and chromatography in order to remove the cellular proteins...
متن کاملDetection of Bovine Viral Diarrhea Virus in Bovine Semen Using Nested-PCR
A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...
متن کامل